Solid-Phase Extraction
All analytical procedures include three steps: extraction, purification, and determination. The most significant recent improvement in the purification step is the use of solid-phase extraction.
Test extracts are cleaned up before instrumental analysis (thin layer or liquid chromatography) to remove coextracted materials that often interfere with the determination of target analytes.
High-pressure Liquid Chromatograph
High-pressure Liquid Chromatography (HPLC) is widely used as a separation technique in aflatoxin analysis to separate, identify, and quantify compounds. It includes analyte application, stationary phase, and mobile phase.
Liquid chromatography methods for the determination of aflatoxins in foods include normal-phase LC (NPLC), reversed-phase LC (RPLC) with pre- or before-column derivatization (BCD), RPLC followed by postcolumn derivatization (PCD), and RPLC with electrochemical detection.
Immunochemical Methods
HPLC methods for determining aflatoxins in food are laborious and time consuming. These techniques often require knowledge and experience of chromatographic techniques to solve sepatation and and interference problems. Through advances in biotechnology, highly specific antibody-based tests are now commercially available that can identify and measure aflatoxins in food in less than 10 minutes. These tests are based on the affinities of the monoclonal or polyclonal antibodies for aflatoxins. The three types of immunochemical methods are radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), and immunoaffinity column assay (ICA).
http://www.fs-pt.com/analytical_laboratory_services/aflatoxin_aspergillus_analysis.html
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